Not known Facts About attune flow cytometer
Not known Facts About attune flow cytometer
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Facts-enriched characterization of mobile populations. Morphological facts from photos can incorporate for the richness of flow cytometry details. For example, the figure shows an normally regular apoptosis assay using Annexin V and PI, introducing cell imaging to characterize cells in Each individual populace to reveal morphologically unique options. These insights could not are already received from multiplex staining by itself.
As samples are acquired over the Attune CytPix Flow Cytometer, the higher-pace brightfield digicam captures and shops pictures of detected functions in a price nearly 6,000 visuals for every 2nd, based on the flow rate and impression dimension. For larger flexibility, Attune Cytometric Software package permits you to regulate the image capture frequency as wanted and choose a certain gate for image capture, if demanded.
(B) RAW 264.seven macrophages are sometimes annotated inaccurately with respect to particle depend because of their complexity and noncircular borders. The number of singlets available for Investigation raises as soon as the consumer trains and employs a far more accurate model.
Uncover new insights into your experimental details by visualizing populations utilizing put together fluorescence and morphology details. Our software package interface wizards allow downsampling, concatenation (merged data files) and dimensionality resources which might be developed into your program for an intuitive, streamlined facts analysis practical experience.
"An image just isn't pretty truly worth one thousand dots, but it definitely helps to see photographs. It is excellent to see photos that correlate with staining. For example, AnnexinV-positive cells which might be morphologically diverse than healthful cells, or CD14+ monocytes which have been greater plus more textured than CD3+/CD4+/CD14- lymphocytes.
Moreover, it can be illuminating to discover the number of doublets slip through regular singlet gates, and terrific to confirm the status of unpredicted double-beneficial events in my Investigation."
Pair your Attune flow cytometer with the autosampler for greater workflow efficiency, and add robotic integration for optimum throughput.
Steady picture high quality even at superior throughput Acoustic concentrating plus a substantial-speed digital camera Incorporate to picture these T cells continuously at small or higher flow fees. Easily change target and digital camera settings to satisfy experimental needs.
The Attune NxT cytometer would be the reputable workhorse instrument your lab can depend upon for constant and reliable general performance. The Attune CytPix product incorporates a superior-velocity brightfield digital camera and AI-driven picture Assessment software package, with recent updates together with device Finding out tools and an intuitive person interface, enabling Increased details precision, precision, and quicker insights into sample biology.
Probably most significantly, these gatherings have cells of clearly distinct phenotypes which could lead on to incorrect conclusions regarding double good activities (specifically in scarce populations).
AI-driven automatic impression Evaluation Along with the Attune Cytometric Program interprets party functions into distinctive morphology parameters that can be coupled with normal fluorescence and scatter parameters to expose really reproducible population modifications and exclusive subsets.
We previously shown the strength of imaging Automobile-T/Ramos cell interactions. Allow’s take a look at just the population of finest fascination, the double constructive functions, to learn more. We could now use extended morphological parameters (listed here, circularity and skewness of depth) to even further take a look at the options of these populations and refine gating on these events, escalating knowledge robustness.
Created-in dimensionality reduction instruments improved resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to substantiate predicted inhabitants resolution in lysed total human blood. User can perspective situations from Every single cluster for visual confirmation of mobile phenotypes.
Versatile payment program from Agilent Fiscal Options enable you to have the critical instrumentation you may need for instant task, or to expand your lab.
Gating on these prolonged parameters permits you to immediately and correctly detect populations of interest and ensure gating strategy with little if any handbook overview. The Attune program offers usage of condition-of-the-artwork device Finding out Examination equipment inside of an expertly made consumer interface, more info making it exceptionally simple to use.
As the cell line experienced previously been analyzed to the Attune CytPix Flow Cytometer, the researchers went again to the photographs and were ready to doc the microbial an infection a minimum of five days before.
The Attune CytPix Flow Cytometer permits customers to visualise samples during acquisition, confirm the precision of gating strategies, and assistance effects with responsible documentation for publication applications.
Right here, we demonstrate that by gating procedures working with image-centered quantitative parameters can distinguish interacting cells from coincident occasions to much more correctly evaluate interacting cells.